eCM Meeting Abstracts 2012, Collection 4
Abstracts of the Tissue and Cell Engineering Society (TCES)
meeting
4-6 July 2012, University of Liverpool
All abstracts: click here to download all the abstracts in one file
Page 1: LA Bosworth, SR Rathbone, S Downes, SH Cartmell
Cyclical loading of electrospun scaffolds affects mesenchymal stem cell response
Page 2: S Khan, SM Richardson, JA Hoyland
Interactions between mesenchymal stem cells and nucleus pulposus cells: Implications for intervertebral disc regeneration
Page 3: P Sawadkar, S Alexander, M Tolk, L Bozec, V Mudera
Testing and FEA modelling of a modified suture technique to accommodate a tissue engineered tendon in vivo
Page 4: NM Hopper, J Wardale, N Rushton
Mononuclear cells enhance cell migration out of human articular cartilage
Page 5: JHA Bell, JW Haycock
Investigating the effect of plasma polymers on neuronal and glial cells
Page 6: MFB Daud, KC Pawar, F Claeyssens, AJ Ryan, JW Haycock
The development of a 3D neuronal glial co-culture model using aligned electrospun polycaprolactone (PCL) microfibres scaffolds for peripheral nerve studies
Page 7: ML Vermeille, DF Kalbermatten, W Raffoul, M Wiberg, PJ Kingham
Injection of adipose derived stem cells after peripheral nerve injury: mechanisms and effects on regeneration
Page 8: AP Hopper, F Claeyssens
Amine functionalised nanodiamond as a neuronal cell substrate
Page 9: ET Osei, L Evans, JB Phillips, AJ Loughlin
Developing a 3D co-culture model to simulate perinatal hypoxic-ischaemic brain injury in preterm infants
Page 10: MK Kolar, LN Novikova, M Wiberg, LN Novikov, PJ Kingham
Transplantation of human adipose derived stem cells reduces glial cell reactions after spinal cord injury in rats
Page 11: P Deshpande, C Ramachandran, F Sefat, C Johnson, I Mariappan, D Balasubramanian, AJ Ryan, G Vemuganti, VS Sangwan, S MacNeil
Development of synthetic scaffolds for delivering limbal epithelial cells to the cornea
Page 12: I Ortega, P Deshpande, AJ Ryan, S MacNeil, F Claeyssens
Development of tissue engineered stem cell niches for corneal repair
Page 13: SL Mason, SB Kaye, P Rooney, E Austin, CM Sheridan
Time in organ culture storage has a detrimental effect on the survival of limbal cells
Page 14: K Stamati, V Mudera, U Cheema
Stem cell generated angiogenic growth factor gradient: guiding endothelial cells
Page 15: EG Knight, SA Przyborski
Neural differentiation of pluripotent stem cells is regulated by scaffold format
Page 16: M Fok, N Bryan, J Hunt
Immunological considerations of autologous stem cell transplantation following immuno-affinity separation
Page 17: E Kingham, N Gadegaard, MJ Dalby, ROC Oreffo
Nanotopography-induced osteogenic differentiation of human embryonic stem cells and adult skeletal stem cells
Page 18: PM Tsimbouri, N Gadegaard, E Kingham, ROC Oreffo, MJ Dalby
Mechanotransduction effects and stem cell differentiation in response to bioactive nanotopography
Page 19: HT van Veen, F Pu, NP Rhodes, JA Hunt
Mononuclear cells for haematopoietic progenitor cell expansion as an approach to increase output for erythroid maturation
Page 20: FC Lewis, N Bryan, D Bond, C Stanley, JA Hunt
Evaluation of a novel, rapid method to catch and release therapeutic numbers of viable adult stem cells from adipose
Page 21: SL Passey, AST Smith, NRW Martin, V Mudera, L Greensmith, K Baar, MP Lewis
Optimisation of a 3D engineered skeletal muscle-motoneuron co-culture using fibrin-cast gels
Page 22: D Schaakxs, DF Kalbermatten, W Raffoul, M Wiberg, PJ Kingham
Regenerative cell injection in denervated muscle reduces atrophy and enhances recovery following nerve repair
Page 23: FC Lewis, BJ Henning, G Marazzi, D Sassoon, GM Ellison, B Nadal-Ginard
Identification and characterisation of multipotent PW1+ interstitial cells from porcine skeletal muscle
Page 24: N Bryan, H Ashwin, Y Bayon, S Wohlert, N Smart, JA Hunt
In vitro measurement of material stimulated leukocyte reactive oxygen species (ROS) production as a predictor of the acute inflammatory reaction in vivo
Page 25: GE Morris, AJ Knox, JW Aylott, AM Ghaemmaghami, CE Brightling, FRAJ Rose
Moving towards a 3D environment for in vitro studies: an alternative to animal studies in asthma research
Page 26: L Partington, NJ Mordan, J Knowles, MW Lowdell, M Birchall, I Wall
Biochemical changes caused by decellularization may compromise mechanical integrity of tracheal scaffolds
Page 27: NS Tan, T Alekseeva, RA Brown
Semi-quantitative method for collagen density analysis: use in rapid tissue fabrication
Page 28: LJ White, MJ Sawkins, SF Badylak, KM Shakesheff
Decellularised bone gels – novel materials for bone regeneration
Page 29: RS Alazragi, RPW Davies, S Russell, A Aggeli
A novel dressing coated with self-assembled peptide for wound healing
Page 30: C Hamilton, CJ Henderson, RV Ulijn, MH Grant
Galactosylated poly (ethylene glycol) as a scaffold for primary hepatocytes
Page 31: H Yang, E Chruscikowski, J Segal, S Ratchev
Hydraulic and rheological evaluation of a pneumatic direct-write bioprinter
Page 32: I Paik, DJ Scurr, B Morris, G Hall, C Denning, MR Alexander, KM Shakesheff, JE Dixon
Rapid micropatterning of cell lines and human pluripotent stem cells on elastomeric membranes
Page 33: I Wimpenny, Y Yang, AJ El Haj
Fabrication and degradation of fluorescently tagged 3D scaffolds
Page 34: CM Nickson, S Dixon, RL Williams
Endothelial cell growth on novel biocompatible polymeric coatings, for potential use as cardiovascular stent coatings
Page 35: LE Sidney, A Abed, TRJ Heathman, CV Rahman, LDK Buttery
Investigation of anti-inflammatory drug delivery from polymeric scaffolds using an in vitro bone inflammation model
Page 36: MJ Sawkins, BN Brown, LJ Bonassar, FRAJ Rose, KM Shakesheff
Bioplotting of novel scaffold materials and complex constructs for osteochondral tissue engineering
Page 37: R Balint, NJ Cassidy, SH Cartmell
Osteogenic differentiation of primary human Mesenchymal Stem Cells enhanced by capacitive electrical stimulation
Page 38: PJM Wilson, JP Dillon, BW Wlodarski, WD Fraser, SC Wagstaff, JA Gallagher
Expression of type VI collagen in bone and osteosarcoma cell lines indicates a role in the development of bone
Page 39: SA Fawcett, L Hammilton, JM Curran, NP Rhodes, MR Alexander, K Shakesheff, JA Hunt
Single and multiple surface chemical modifications applied to a 3D PLGA scaffold for mesenchymal stem cell differentiation in vitro
Page 40: LE McNamara, T Sjöström, K Seunarine, K Burgess, ROC Oreffo, RM Dominic Meek, B Su, MJ Dalby
Osteogenic nano- and multi-structured titania surfaces for orthopaedics
Page 41: RJ McMurray, J Connelly, MM Knight
Topographic regulation of primary cilia length and orientation in mesenchymal stem cells
Page 42: A Doyle, S Crosby, DR Burton, F Lilley, M Murphy
The effects of elongation factor 1 alpha (eEF1A) on the organisation of actin and the mechanical properties of yeast cells
Posters
Page 43: M Fok, N Bryan, J Hunt
A novel method for isolation and release of autologous adult stem cells from primary tissue
Page 44: TF Somerville, S Ahmad, SB Kaye, CM Sheridan, RMK Stewart
Viability of porcine and bovine corneal endothelium in culture
Page 45: CL Dong, WR Webb, Q Peng, JZ Tang, NR Forsyth, GQ Chen, AJ El Haj
Development of platelet derived growth factor-ββ release PHBHHx nanoparticles to control differentiation of human mesenchymal stem cells
Page 46: D Gothard, J Kanczler, K Cheung, ROC Oreffo
Isolated human foetal femur cells maintain Stro-1 expression and differentiation potential over prolonged culture: a potential source of skeletal stem cells
Page 47: A Alraies, RJ Waddington, R Moseley, AJ Sloan
Variations in dental pulp progenitor cell ageing influence regenerative potential
Page 48: M Peca, D Gothard, H Morgan, ROC Oreffo
High-throughput skeletal stem cell separation using magnetic labelling and microfluidic sorting
Page 49: H Nikukar, S Reid, M Riehle, AS Curtis, MJ Dalby
Nanomechanotransduction of human mesenchymal stem cell
Page 50: EV Alakpa, V Jayawarna, K Burgess, R Ulijn, M Dalby
Characterisation of peptide biomaterials & innate metabolites that direct stem cell differentiation in vitro
Page 51: Z Nie, H-Y Lee, H-W Kim, M Hoare, IB Wall
Establishment of a bioprocess step to enhance cell-based bone regeneration
Page 52: A Dunham, XT Yan, MH Grant
An investigation of the stiffness moduli of artificial bone materials
Page 53: LA Castillo, A Saiani, JE Gough, AF Miller
Design of functional octa-peptide to direct bone formation
Page 54: N Johns, E East, JP Golding, AJ Loughlin, JB Phillips
Astrocytes expressing GFP in 3D collagen gels provide an effective model for screening the glial response to potential CNS cell therapies
Page 55: CM O’Rourke, AJ Loughlin, R Drake, JB Phillips
Defining glial cell self-alignment parameters for 3D CNS tissue models
Page 56: JW Thwaites, N Habib, P Dalby, I Wall
Impact of cell seeding density on early neuronal differentiation of human pluripotent cells
Page 57: M Georgiou, J Loughlin, JP Golding, PJ Kingham, JB Phillips
Engineered neural tissue with aligned Schwann cells supports neuronal regeneration in vivo and can be assembled using differentiated adipose-derived stem cells
Page 58: A Ardakani, RJ Shipley, K Stamati U Cheema, RA Brown
Quantifying spatial and temporal variation in cell function in collagen constructs for tissue engineering applications
Page 59: W Chrzanowski, S Yamaguchi, N Mordan, V Salih, T Kokubo
Gummetal – a new implantable metal with chemical and mechanical biocompatibility
Page 60: S Dunphy, H Dua, A El Haj, A Hopkinson, F Rose
Development of microfibrous and nanofibrous PLGA electrospun scaffolds: Strategies in corneal tissue engineering
Page 61: FR Pu, NP Rhodes, Y Bayon, R Chen, O Lefranc, P Gravagna, G Brans, R Benne, JA Hunt
Abdominal wall reconstruction using novel PLLA-collagen scaffolds
Page 62: MS Purcell, E Tayton, RO Oreffo, KM Shakesheff, SM Howdle
Supercritical fluid foaming: a novel route to polymeric allografts?
Page 63: W Chrzanowski, I Raizer, N Mordan, JC Knowles, V Salih
Characterisation of ceramic - filled fibrous polylactide membranes for
cell engineering applications
Page 64: H Ashwin, J Hunt, E Mausy, J Migliozzi, D Beers, S Wohlert
Influence of varying HMDI crosslinking parameters on porcine dermis
Page 65: H Ashwin, N Bryan, S Wohlert, J Hunt
In vivo and in vitro assessment of foreign body reaction to HMDI crosslinked porcine dermis
Page 66: M Navarro, L Nelson, O Tura, K Samuel, P Hayes, J Plevris
Defining in vitro co-culture systems for human liver tissue engineering
Page 67: CM Rogers, S Toumpaniari, R Bail, J Segal, KM Shakesheff, FRAJ Rose
Developing electrospun scaffolds with tailored geometries
Page 68: HT Gilbert, IA Barker, S Leigh, JA Covington, AP Dove, JA Hoyland, SM Richardson
Production of dipentaerythritol-poly(ethylene glycol) acrylate based constructs via microsteriolithography for cell-based tissue engineering
Page 69: S Wan, A Saiani, S Richardson, J Gough
Self-assembling peptide gels for intervertebral disc tissue engineering
Page 70: K Luetchford, J Chaudhuri, P De Bank
Processing silk-based naturally derived materials for three-dimensional cell culture and tissue engineering applications
Page 71: JB Rose, DJ Williams, A El Haj, H Dua, A Hopkinson, FRAJ Rose
Evaluation of electrospun gelatin/ polycaprolactone as a potential artificial corneal stroma
Page 72: S Roman, N Osman, A Mangera, AJ Bullock, CR Chapple, S MacNeil
Developing an autologous engineered connective tissue using a biodegradable scaffold for the treatment of stress urinary incontinence and pelvic organ prolapse
Page 73: F Sefat, P Deshpande, C Ramachandran, I Mariappan, D Balasubramanian, G Vemuganti, AJ Ryan, VS Sangwan, S MacNeil
Development and characterisation of biodegradable PLGA scaffolds for delivering corneal limbal stem cells to damaged corneas
Page 74: A Raza, E Baggaley, JA Weinstein, S MacNeil, JW Haycock
Development of a simple 3D melanoma skin model incorporating Pt (II) labels
and two photon / time-resolved emission imaging microscopy for detection
Page 75: A Chhatwal, R Mold, S Przyborski
Continual propagation of cells in three-dimensional culture
Page 76: DJ Player, S Passey, V Mudera, MP Lewis
Development of tissue engineered skeletal muscle constructs for nutritional supplement testing
Page 77: AJ Taylor, MR Alexander, LDK Buttery
The production and characterisation of primary calvarial cell-secreted decellularised matrices
Page 78: AC Harvey, SP Armes, CWI Douglas, S MacNeil
Biologically active copolymer hydrogels
Page 79: TWA Gould, AS Mallick, JP Birchall, LR Lustig, KM Shakesheff, CV Rahman
Development of a porous polymer scaffold for mastoid bone regeneration
Page 80: J Wong, AJ MacRobert, U Cheema, RA Brown
Engineering anisotropy in mechanical properties by orientated collagen cross-linking
Page 81: L Szkolar, JE Gough, A Saiani
The development of functional peptide scaffolds for cell culture
Page 82: HC Cox, LJ White, Y Reinwald, GTS Kirby, FRAJ Rose, KM Shakesheff
Sustained delivery of active BMP-2 from PLGA microspheres for bone regeneration applications
Page 83: JC Guedes, N Lakhkar, JC Knowles, I Wall
Assessment of TiO2-doped bioactive glass microcarriers for expansion of adherent mammalian cells
Page 84: F Gesellchen, AL Bernassau, DRS Cumming, MO Riehle
Cell manipulation using an acoustic tweezing device – Application in cell patterning and adhesion testing
Page 85: M McCully, H Child, MJ Dalby, CC Berry
Using functionisled gold nanoparticles for the delivery of siRNA to bone cancer cells in vitro
Page 86: NI Osman, S Roman, AJ Bullock, CR Chapple, S MacNeil
Improving the elasticity of an autologous tissue engineered prosthesis (TEP) for pelvic floor repair by the use of mechanical stimulation
Page 87: EL Smith, CA Roberts, JM Kanczler, ROC Oreffo
Elucidating skeletal development to inform bone tissue regeneration – lessons from ex vivo chick femur cultures
Page 88: S Puwanun, F Bye, RM Delaine-Smith, J Yates, S MacNeil, GC Reilly
Optimizing cell sources and materials for cleft palate repair
Page 89: N Bryan, H Ashwin, R Chen, Y Bayon, S Wohlert, N Smart, JA Hunt
In vivo evaluation of the influence of processing variables in the down-stream inflammation elicited by xenogeneic soft tissue repair biomaterials
Page 90: CM Keenan, CE Edwards, AJ Preston, H Sutherland, B Wlodarski, AM Taylor, D Williams, LR Ranganath, JA Gallagher, JC Jarvis
The appearance and distribution of ochronpsis in the calcified cartilage of a murine model of alkaptonuria
Page 91: C Platt, AM Taylor, JC Jarvis, LR Ranaganth, A Boyde, JA Gallagher
Is “Frostian” bone remodelling the dominant mechanism of altering bone microarchitecture in ageing human bone?
Page 92: RL Adams, SA Przyborski
Developing a novel 3D migration model to study colon cancer cell invasion
Page 93: VR Kearns, K Vasilev, CM Sheridan, RL Williams
The influence of surface chemistry on ECM production by retinal pigment epithelial (hRPE) cells on expanded polytetrafluoroethylene (ePTFE) substrates
Page 94: H Peto, L Buttery, K Shakesheff
System for expressing high quantities of growth factors in E.Coli in soluble form
Page 95: L O’Donovan, P De Bank
A novel caged carbonyl for biomaterial modification and cell patterning
Page 96: I Benzeval, IG Turner, MJ Ellis
Adhesion strength of the MG63 cell line and its applicability to a fluidised bed bioreactor
Page 97: NJ Lakhkar, JH Park, V Salih, JC Knowles
Titanium phosphate glass microspheres: novel microcarriers for bioreactor-mediated bone cell scale-up and differentiation
Page 98: SR Tew, McDermott B, Clegg PD
Regulation of SOX9 in human chondrocytes by the RNA binding protein tristetraprolin
Page 99: LAC Chapman, SL Waters, RJ Shipley, HM Byrne, JP Whiteley, MJ Ellis
Modelling fluid and nutrient transport to determine the influence of cell seeding on the growth of cell aggregates on a permeable membrane
Page 100: LE Sidney, GR Kirkham, LDK Buttery
The effect of an inflammatory environment on the response and differentiation of osteogenic embryonic stem cells
All abstracts: click here to download all the abstracts in one file